In vitro and in vivo studies of the endocrine disrupting potency of cadmium in roach (Rutilus rutilus) liver.
Identifieur interne : 000177 ( Main/Exploration ); précédent : 000176; suivant : 000178In vitro and in vivo studies of the endocrine disrupting potency of cadmium in roach (Rutilus rutilus) liver.
Auteurs : M. Gerbron [France] ; P. Geraudie [Norvège] ; B. Xuereb [France] ; S. Marie [France] ; C. Minier [France]Source :
- Marine pollution bulletin [ 1879-3363 ] ; 2015.
English descriptors
- KwdEn :
- Animals, Cadmium (metabolism), Cadmium (toxicity), Cyprinidae (physiology), Endocrine Disruptors (metabolism), Endocrine Disruptors (toxicity), Estradiol (metabolism), Estradiol (toxicity), Estrogen Receptor alpha (metabolism), In Vitro Techniques, Liver (metabolism), RNA, Messenger (metabolism), Receptors, Androgen (metabolism), Receptors, Estrogen (metabolism), Toxicity Tests, Vitellogenins (metabolism), Water Pollutants, Chemical (metabolism), Water Pollutants, Chemical (toxicity).
- MESH :
- chemical , metabolism : Cadmium, Endocrine Disruptors, Estradiol, Estrogen Receptor alpha, RNA, Messenger, Receptors, Androgen, Receptors, Estrogen, Vitellogenins, Water Pollutants, Chemical.
- chemical , toxicity : Cadmium, Endocrine Disruptors, Estradiol, Water Pollutants, Chemical.
- metabolism : Liver.
- physiology : Cyprinidae.
- Animals, In Vitro Techniques, Toxicity Tests.
Abstract
Cadmium has been reported to exert estrogenic, antiestrogenic or both effects in vertebrate species. To elucidate the endocrine disrupting action of CdCl2, ex vivo and in vivo experiments were performed in roach (Rutilus rutilus). Roach liver explants were exposed to a range of CdCl2 concentrations alone (0.1-50μM) or with an effective concentration (100nM) of 17β-estradiol (E2). In addition, juvenile roach were intraperitoneally injected with CdCl2 (0.1-2.5mg/kg) with or without 1mg E2/kg. Subsequent analysis evaluated the effect of CdCl2 on vitellogenin (VTG) synthesis both at the mRNA and protein level, on estrogen receptors (erα and erβ1) and on androgen receptor (ar) mRNA expression. Ex vivo and in vivo experiments indicated that CdCl2 is strongly anti-estrogenic as, when co-exposed to E2, CdCl2 significantly inhibited VTG production as well as vtg and erα mRNA expressions. Moreover, CdCl2 compromised the E2-mediated induction of the ar mRNA expression in vivo.
DOI: 10.1016/j.marpolbul.2015.03.043
PubMed: 26024563
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000078
- to stream PubMed, to step Curation: 000078
- to stream PubMed, to step Checkpoint: 000078
- to stream Ncbi, to step Merge: 000474
- to stream Ncbi, to step Curation: 000474
- to stream Ncbi, to step Checkpoint: 000474
- to stream Main, to step Merge: 000175
- to stream Main, to step Curation: 000177
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">In vitro and in vivo studies of the endocrine disrupting potency of cadmium in roach (Rutilus rutilus) liver.</title>
<author><name sortKey="Gerbron, M" sort="Gerbron, M" uniqKey="Gerbron M" first="M" last="Gerbron">M. Gerbron</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre, France. Electronic address: marie.gerbron@univ-lehavre.fr.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre</wicri:regionArea>
<placeName><region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Le Havre</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Geraudie, P" sort="Geraudie, P" uniqKey="Geraudie P" first="P" last="Geraudie">P. Geraudie</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre, France; Akvaplan Niva as, Fram Centre, 9296 Tromsø, Norway.</nlm:affiliation>
<country xml:lang="fr">Norvège</country>
<wicri:regionArea>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre, France; Akvaplan Niva as, Fram Centre, 9296 Tromsø</wicri:regionArea>
<wicri:noRegion>9296 Tromsø</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xuereb, B" sort="Xuereb, B" uniqKey="Xuereb B" first="B" last="Xuereb">B. Xuereb</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre</wicri:regionArea>
<placeName><region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Le Havre</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Marie, S" sort="Marie, S" uniqKey="Marie S" first="S" last="Marie">S. Marie</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre</wicri:regionArea>
<placeName><region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Le Havre</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Minier, C" sort="Minier, C" uniqKey="Minier C" first="C" last="Minier">C. Minier</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre</wicri:regionArea>
<placeName><region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Le Havre</settlement>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2015">2015</date>
<idno type="RBID">pubmed:26024563</idno>
<idno type="pmid">26024563</idno>
<idno type="doi">10.1016/j.marpolbul.2015.03.043</idno>
<idno type="wicri:Area/PubMed/Corpus">000078</idno>
<idno type="wicri:Area/PubMed/Curation">000078</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000078</idno>
<idno type="wicri:Area/Ncbi/Merge">000474</idno>
<idno type="wicri:Area/Ncbi/Curation">000474</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000474</idno>
<idno type="wicri:Area/Main/Merge">000175</idno>
<idno type="wicri:Area/Main/Curation">000177</idno>
<idno type="wicri:Area/Main/Exploration">000177</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">In vitro and in vivo studies of the endocrine disrupting potency of cadmium in roach (Rutilus rutilus) liver.</title>
<author><name sortKey="Gerbron, M" sort="Gerbron, M" uniqKey="Gerbron M" first="M" last="Gerbron">M. Gerbron</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre, France. Electronic address: marie.gerbron@univ-lehavre.fr.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre</wicri:regionArea>
<placeName><region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Le Havre</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Geraudie, P" sort="Geraudie, P" uniqKey="Geraudie P" first="P" last="Geraudie">P. Geraudie</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre, France; Akvaplan Niva as, Fram Centre, 9296 Tromsø, Norway.</nlm:affiliation>
<country xml:lang="fr">Norvège</country>
<wicri:regionArea>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre, France; Akvaplan Niva as, Fram Centre, 9296 Tromsø</wicri:regionArea>
<wicri:noRegion>9296 Tromsø</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xuereb, B" sort="Xuereb, B" uniqKey="Xuereb B" first="B" last="Xuereb">B. Xuereb</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre</wicri:regionArea>
<placeName><region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Le Havre</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Marie, S" sort="Marie, S" uniqKey="Marie S" first="S" last="Marie">S. Marie</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre</wicri:regionArea>
<placeName><region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Le Havre</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Minier, C" sort="Minier, C" uniqKey="Minier C" first="C" last="Minier">C. Minier</name>
<affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratory of Ecotoxicology, ULH Normandie Université, SFR SACLE 4116, BP 540, 76058 Le Havre</wicri:regionArea>
<placeName><region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Le Havre</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Marine pollution bulletin</title>
<idno type="eISSN">1879-3363</idno>
<imprint><date when="2015" type="published">2015</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Cadmium (metabolism)</term>
<term>Cadmium (toxicity)</term>
<term>Cyprinidae (physiology)</term>
<term>Endocrine Disruptors (metabolism)</term>
<term>Endocrine Disruptors (toxicity)</term>
<term>Estradiol (metabolism)</term>
<term>Estradiol (toxicity)</term>
<term>Estrogen Receptor alpha (metabolism)</term>
<term>In Vitro Techniques</term>
<term>Liver (metabolism)</term>
<term>RNA, Messenger (metabolism)</term>
<term>Receptors, Androgen (metabolism)</term>
<term>Receptors, Estrogen (metabolism)</term>
<term>Toxicity Tests</term>
<term>Vitellogenins (metabolism)</term>
<term>Water Pollutants, Chemical (metabolism)</term>
<term>Water Pollutants, Chemical (toxicity)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cadmium</term>
<term>Endocrine Disruptors</term>
<term>Estradiol</term>
<term>Estrogen Receptor alpha</term>
<term>RNA, Messenger</term>
<term>Receptors, Androgen</term>
<term>Receptors, Estrogen</term>
<term>Vitellogenins</term>
<term>Water Pollutants, Chemical</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Cadmium</term>
<term>Endocrine Disruptors</term>
<term>Estradiol</term>
<term>Water Pollutants, Chemical</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Liver</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cyprinidae</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>In Vitro Techniques</term>
<term>Toxicity Tests</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Cadmium has been reported to exert estrogenic, antiestrogenic or both effects in vertebrate species. To elucidate the endocrine disrupting action of CdCl2, ex vivo and in vivo experiments were performed in roach (Rutilus rutilus). Roach liver explants were exposed to a range of CdCl2 concentrations alone (0.1-50μM) or with an effective concentration (100nM) of 17β-estradiol (E2). In addition, juvenile roach were intraperitoneally injected with CdCl2 (0.1-2.5mg/kg) with or without 1mg E2/kg. Subsequent analysis evaluated the effect of CdCl2 on vitellogenin (VTG) synthesis both at the mRNA and protein level, on estrogen receptors (erα and erβ1) and on androgen receptor (ar) mRNA expression. Ex vivo and in vivo experiments indicated that CdCl2 is strongly anti-estrogenic as, when co-exposed to E2, CdCl2 significantly inhibited VTG production as well as vtg and erα mRNA expressions. Moreover, CdCl2 compromised the E2-mediated induction of the ar mRNA expression in vivo.</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
<li>Norvège</li>
</country>
<region><li>Haute-Normandie</li>
<li>Région Normandie</li>
</region>
<settlement><li>Le Havre</li>
</settlement>
</list>
<tree><country name="France"><region name="Région Normandie"><name sortKey="Gerbron, M" sort="Gerbron, M" uniqKey="Gerbron M" first="M" last="Gerbron">M. Gerbron</name>
</region>
<name sortKey="Marie, S" sort="Marie, S" uniqKey="Marie S" first="S" last="Marie">S. Marie</name>
<name sortKey="Minier, C" sort="Minier, C" uniqKey="Minier C" first="C" last="Minier">C. Minier</name>
<name sortKey="Xuereb, B" sort="Xuereb, B" uniqKey="Xuereb B" first="B" last="Xuereb">B. Xuereb</name>
</country>
<country name="Norvège"><noRegion><name sortKey="Geraudie, P" sort="Geraudie, P" uniqKey="Geraudie P" first="P" last="Geraudie">P. Geraudie</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/France/explor/LeHavreV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000177 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000177 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/France |area= LeHavreV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:26024563 |texte= In vitro and in vivo studies of the endocrine disrupting potency of cadmium in roach (Rutilus rutilus) liver. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26024563" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a LeHavreV1
This area was generated with Dilib version V0.6.25. |